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MS Imaging
Developments in MS Imaging
MALDI MS Imaging (MALDI MSI) is a technology providing access to the 2D distribution or 3D volume reconstruction of endogenous and exogenous compounds from ex vivo thin tissue sections. MALDI MSI shows several advantages including- Access to the distribution of hundreds molecules in one acquisition.
- Access to various classes of endogeneous molecules (metabolites, lipids, peptides & proteins) or exogenous molecules (drugs, xenobiotics)
- No need of prerequisite knowledge on the molecules to study
- No need to use tagged or probes molecules
Download Related Publications to MALDI MS Imaging- R. Lemaire, M. Wisztorski, A. Desmons, J.C. Tabet, R. Day, M. Salzet, I. Fournier
Anal. Chem. (2006) 78, 7145-7153
MALDI-MS Direct Tissue Analysis of Proteins : Improving Signal Sensitivity Using Organic Treatments
Download PDF - M. Wisztorski, R. Lemaire, J. Stauber, S. Aït-Menguellet, D. Croix, O. Jardin-Mathé, R. Day, M.Salzet, I. Fournier
Curr. Pharm. Design (2007) 13(32), 3317-3324
New developments in MALDI Imaging for Pathology Proteomics Studies
Download PDF - I. Fournier, J. Franck, M. Wisztorski, E. Macagno, M. Salzet
Neurosci. Imaging (2008) 3(1), 19-32
MALDI imaging : a Review of the Current Status of the Technology
Download PDF - M. Wisztorski, M. Salzet, I. Fournier
Expert Review Proteomics (2008) 5(3), 413-24
Tissue Imaging Using MALDI Mass Spectrometry : The New Frontier of Histopathology Proteomics
Download PDF - Franck J., Arafah K., Elayed M., Bonnel D., Vergara D., Jacquet A., Vinatier D., Wisztorski M., Day R., Fournier I., Salzet M.
Mol Cell Proteomics (2009) 8(9), 2023-33
MALDI imaging mass spectrometry : state of the art technology in clinical proteomics
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We have at CLIC Imaging Core Facility a long knowledge in the MALDI MS Imaging technology and been involved in the developments of several improvements and strategies for the technology
Ionic Matrixes
Matrix is a central piece of the MALDI desorption/ionization process.
We have develop new matrixes dedicated to MALDI MS Imaging applications namely ionic matrixes. Ionic matrixes are organic salts obtained through acid/base reaction between conventional MALDI matrices and different organic bases. We have developed ionic matrixes for various classes of biomolecules which are either solid (SIMs) or Liquid (LIMs). Selected ionic matrixes show superior results than conventional matrixes for tissue profiling or imaging.
Download Related Publications & Patents
Patents- EP 2 287 602 A1
Use of Ionic Matrices for MALDI Mass Spectrometry Analysis of Tissue Sections
Inventors : I. Fournier, R. Lemaire, J.C. Tabet, M. Salzet (inventors)
Applicants : CNRS - Université Lille 1
Download Patent Document - WO 2011/073740 A1
Matrices for Mass Spectrometry Imaging
Inventors : I. Fournier, M. Salzet, C. Meriaux, J. Franck
Applicants : CNRS - Université Lille 1
Download Patent Document
Publications - R. Lemaire, J.C. Tabet, P. Ducoroy, J.B. Hendra, M.Salzet, I. Fournier
Anal. Chem. (2006) 78, 809-819
Solid Ionic Matrices for Direct Tissue Analysis and MALDI Imaging
Download PDF - Franck J., Arafah K., Barnes A., Wisztorski M., Salzet M., Fournier I.
Anal Chem. (2009) 81(19), 8193-202
Improving tissue preparation for matrix-assisted laser desorption ionization mass spectrometry imaging. Part 1 : using microspotting
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Download Supplementary Data - Meriaux C, Franck J, Wisztorski M, Salzet M, Fournier I.
J Proteomics. (2010) 73(6) : 1204-18
Liquid ionic matrixes for MALDI mass spectrometry imaging of lipids
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Download Supplementray Data - Bonnel D, Franck J, Mériaux C, Salzet M, Fournier I.
Anal Biochem. 2013, 434 : 187-198
Ionic matrices pre-spotted MALDI plates for patients markers following, drugs titration and MALDI MSI
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Clinical FFPE Samples
Formalin Fixed & Paraffin Embedded (FFPE) Samples is the world standards for tissue conservation in Hospital for Clinics. Indeed FFPE samples show a peculiarly good stability along the conservation time and superior morphology preservation after sectioning. However, FFPE samples show certain specifies due to the formalin fixation that induces proteins cross-linking within tissue sections. We have developed strategies for studying FFPE samples coming from hospital libraries. Our strategies involved controlled on tissue digestion of proteins.Download Related Publications
- R. Lemaire, A. Desmons, J.C. Tabet R. Day, M. Salzet, I. Fournier
J. Prot. Res. (2007) 6, 1295-1305
Direct Analysis and MALDI Imaging of Formalin Fixed, Paraffin Embedded Tissue Sections
Download PDF - J. Stauber, R. Lemaire, J. Franck, D. Bonnel, D. Croix, R. Day, M. Wisztorski, M. Salzet, I. Fournier
J. Prot Res. (2008) 7(3), 969-978
MALDI Imaging of FFPE Tissues : Application to Model Animals of Parkinson Disease for Biomarker Hunting
Download PDF - Djidja MC, Francese S, Loadman PM, Sutton CW, Scriven P, Claude E, Snel MF, Franck J, Salzet M, Clench MR.
Proteomics. (2009) 9(10) : 2750-63
Detergent addition to tryptic digests and ion mobility separation prior to MS/MS improves peptide yield and protein identification for in situ proteomic investigation of frozen and formalin-fixed paraffin-embedded adenocarcinoma tissue sections
Download PDF - Stauber J, MacAleese L, Franck J, Claude E, Snel M, Kaletas BK, Wiel IM, Wisztorski M, Fournier I, Heeren RM.
J Am Soc Mass Spectrom. (2010) 21(3) : 338-47
On-tissue protein identification and imaging by MALDI-ion mobility mass spectrometry
Download PDF - Wisztorski M., Fatou B., Franck J., desmons A., Farré, I., Leblanc E., Fournier I., Salzet M.
Proteomic Clinical Applications 2013, 7(3-4) : 234-40
Microproteomics by Liquid Extraction Surface Analysis : Application to FFPE tissue to study the Fimbria region of tubo-ovarian cancer
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QUEST-MSI
Development of Quantification Based MS Imaging
QUEST-MSI project aims to set up a novel analytical platform, for spatially-resolved, quantitative, and highly-sensitive analysis of proteins. A new workflow based on spatially resolved protein extraction followed by in-solution processing will allow high confidence identification of a large number of proteins, quantification of identified proteins, and the ability to reconstruct quantitative images of spatial abundance for the identified proteins.
QUEST-MSI: Proof-of-principle experiment of quantitative protein analysis of a half of a rat brain tissue section which demonstrates the complete workflow proposed in this project. Brain section was deposited on a parafilm and pixels were cut-out under microscope using a scalpel. Each pixel was processed separately for protein extraction, reduction/alkylation and trypsin digestion. Tryptic peptides were then separated and analyzed on a LC-ESI system; MS and MS2 data were stored. Recorded data processing allowed us for protein ID and for relative quantification using spectral counting under Scaffold. Image reconstruction was performed in the Microsoft Excel software (Franck et al., 2013).
Download Related Publications
- Franck J., Quanico J., Wisztorski M. , Day R., Salzet M. and Fournier I.
Anal. Chem. 2013, 85: 8127−8134
Quantification Based Mass Spectrometry Imaging of Proteins by Parafilm Assisted Microdissection
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Download Supplementary Information
- Quanico J., Franck J., Dauly C;, Strupat K., Dupuy J., Day R., Salzet M., Fournier I., Wisztorski M.
J. Proteomics 2013, 79: 200-218
Development of Liquid Microjunction Extraction Strategy for Improving Protein Identification from Tissue Sections
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Download Supplementary data
- Wisztorski M., Fatou B., Franck J., desmons A., Farré, I., Leblanc E., Fournier I., Salzet M.
Proteomic Clinical Applications 2013, 7(3-4): 234-40
Microproteomics by Liquid Extraction Surface Analysis: Application to FFPE tissue to study the Fimbria region of tubo-ovarian cancer
Download PDF
