MS Imaging

Developments in MS Imaging

MALDI MS Imaging (MALDI MSI) is a technology providing access to the 2D distribution or 3D volume reconstruction of endogenous and exogenous compounds from ex vivo thin tissue sections. MALDI MSI shows several advantages including
  • Access to the distribution of hundreds molecules in one acquisition.
  • Access to various classes of endogeneous molecules (metabolites, lipids, peptides & proteins) or exogenous molecules (drugs, xenobiotics)
  • No need of prerequisite knowledge on the molecules to study
  • No need to use tagged or probes molecules


Download Related Publications to MALDI MS Imaging

  • R. Lemaire, M. Wisztorski, A. Desmons, J.C. Tabet, R. Day, M. Salzet, I. Fournier
    Anal. Chem. (2006) 78, 7145-7153
    MALDI-MS Direct Tissue Analysis of Proteins : Improving Signal Sensitivity Using Organic Treatments
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  • M. Wisztorski, R. Lemaire, J. Stauber, S. Aït-Menguellet, D. Croix, O. Jardin-Mathé, R. Day, M.Salzet, I. Fournier
    Curr. Pharm. Design (2007) 13(32), 3317-3324
    New developments in MALDI Imaging for Pathology Proteomics Studies
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  • I. Fournier, J. Franck, M. Wisztorski, E. Macagno, M. Salzet
    Neurosci. Imaging (2008) 3(1), 19-32
    MALDI imaging : a Review of the Current Status of the Technology
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  • M. Wisztorski, M. Salzet, I. Fournier
    Expert Review Proteomics (2008) 5(3), 413-24
    Tissue Imaging Using MALDI Mass Spectrometry : The New Frontier of Histopathology Proteomics
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  • Franck J., Arafah K., Elayed M., Bonnel D., Vergara D., Jacquet A., Vinatier D., Wisztorski M., Day R., Fournier I., Salzet M.
    Mol Cell Proteomics (2009) 8(9), 2023-33
    MALDI imaging mass spectrometry : state of the art technology in clinical proteomics
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    We have at CLIC Imaging Core Facility a long knowledge in the MALDI MS Imaging technology and been involved in the developments of several improvements and strategies for the technology

Ionic Matrixes
Matrix is a central piece of the MALDI desorption/ionization process.
We have develop new matrixes dedicated to MALDI MS Imaging applications namely ionic matrixes. Ionic matrixes are organic salts obtained through acid/base reaction between conventional MALDI matrices and different organic bases. We have developed ionic matrixes for various classes of biomolecules which are either solid (SIMs) or Liquid (LIMs). Selected ionic matrixes show superior results than conventional matrixes for tissue profiling or imaging.


Download Related Publications & Patents
Patents

  • EP 2 287 602 A1 Use of Ionic Matrices for MALDI Mass Spectrometry Analysis of Tissue Sections
    Inventors : I. Fournier, R. Lemaire, J.C. Tabet, M. Salzet (inventors)
    Applicants : CNRS - Université Lille 1
    Download Patent Document
  • WO 2011/073740 A1 Matrices for Mass Spectrometry Imaging
    Inventors : I. Fournier, M. Salzet, C. Meriaux, J. Franck
    Applicants : CNRS - Université Lille 1
    Download Patent Document

    Publications
  • R. Lemaire, J.C. Tabet, P. Ducoroy, J.B. Hendra, M.Salzet, I. Fournier
    Anal. Chem. (2006) 78, 809-819
    Solid Ionic Matrices for Direct Tissue Analysis and MALDI Imaging
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  • Franck J., Arafah K., Barnes A., Wisztorski M., Salzet M., Fournier I.
    Anal Chem. (2009) 81(19), 8193-202
    Improving tissue preparation for matrix-assisted laser desorption ionization mass spectrometry imaging. Part 1 : using microspotting
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    Download Supplementary Data
  • Meriaux C, Franck J, Wisztorski M, Salzet M, Fournier I.
    J Proteomics. (2010) 73(6) : 1204-18
    Liquid ionic matrixes for MALDI mass spectrometry imaging of lipids
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    Download Supplementray Data
  • Bonnel D, Franck J, Mériaux C, Salzet M, Fournier I.
    Anal Biochem. 2013, 434 : 187-198
    Ionic matrices pre-spotted MALDI plates for patients markers following, drugs titration and MALDI MSI
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Clinical FFPE Samples
Formalin Fixed & Paraffin Embedded (FFPE) Samples is the world standards for tissue conservation in Hospital for Clinics. Indeed FFPE samples show a peculiarly good stability along the conservation time and superior morphology preservation after sectioning. However, FFPE samples show certain specifies due to the formalin fixation that induces proteins cross-linking within tissue sections. We have developed strategies for studying FFPE samples coming from hospital libraries. Our strategies involved controlled on tissue digestion of proteins.

Download Related Publications

  • R. Lemaire, A. Desmons, J.C. Tabet R. Day, M. Salzet, I. Fournier
    J. Prot. Res. (2007) 6, 1295-1305
    Direct Analysis and MALDI Imaging of Formalin Fixed, Paraffin Embedded Tissue Sections
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  • J. Stauber, R. Lemaire, J. Franck, D. Bonnel, D. Croix, R. Day, M. Wisztorski, M. Salzet, I. Fournier
    J. Prot Res. (2008) 7(3), 969-978
    MALDI Imaging of FFPE Tissues : Application to Model Animals of Parkinson Disease for Biomarker Hunting
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  • Djidja MC, Francese S, Loadman PM, Sutton CW, Scriven P, Claude E, Snel MF, Franck J, Salzet M, Clench MR.
    Proteomics. (2009) 9(10) : 2750-63
    Detergent addition to tryptic digests and ion mobility separation prior to MS/MS improves peptide yield and protein identification for in situ proteomic investigation of frozen and formalin-fixed paraffin-embedded adenocarcinoma tissue sections
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  • Stauber J, MacAleese L, Franck J, Claude E, Snel M, Kaletas BK, Wiel IM, Wisztorski M, Fournier I, Heeren RM.
    J Am Soc Mass Spectrom. (2010) 21(3) : 338-47
    On-tissue protein identification and imaging by MALDI-ion mobility mass spectrometry
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  • Wisztorski M., Fatou B., Franck J., desmons A., Farré, I., Leblanc E., Fournier I., Salzet M.
    Proteomic Clinical Applications 2013, 7(3-4) : 234-40
    Microproteomics by Liquid Extraction Surface Analysis : Application to FFPE tissue to study the Fimbria region of tubo-ovarian cancer
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